L’anticorps Mouton Polyclonal anti-ALDOA a été validé pour WB, IF, IP, EIA, ID, RIA et DB. Il convient pour détecter ALDOA dans des échantillons de Lapin.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Recognizes Aldolase.
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Immunogène
Aldolase isolated and purified from Rabbit muscle. Freund's complete adjuvant is used in the first step of the immunization procedure.
ALDOA
Reactivité: Humain, Souris, Rat
WB, ELISA, IHC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore by adding 1.0 mL of sterile distilled water.
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins.
Agent conservateur
Without preservative
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Antigène
ALDOA
(Aldolase A, Fructose-Bisphosphate (ALDOA))
Sujet
This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.Synonyms: ALDA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, NY-LU-1